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iga antibody, anti-human  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec iga antibody, anti-human
    Iga Antibody, Anti Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iga/IgA+Antibody%2C+anti-human/custom%40130-114-001%4042575315
    Average 96 stars, based on 350 article reviews
    iga antibody, anti-human - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Site-specific profiling of structure and function of Igµ B cell receptor glycans.
    Article Snippet: Prior to B cell sorting, B cells were enriched from Buffy coats using magnetic assisted cell sorting B cell isolation kit II (Miltenyi Biotic, 130-091-151). .. Post enrichment, B cells were stained with Live/Dead Aqua (1:8000) (L34966, ThermoFisher) and CD3 (1:100) (Biolegend, #300488), CD14 (1:100) (Biolegend, #301841), CD19 (1:50) (#557791), CD20 (1:300) (Biolegend, #302332), CD27 (1:60) (Biolegend, #562513), IgD (1:20) (Biolegend, #348206), IgG (1:150) (Biolegend, #564230) and IgA (1:800) (Miltenyi Biotic, #130-113-477) antibodies for 30 minutes on ice in the dark. ..

    Article Title: Gastrointestinal germinal center B cell depletion and reduction in IgA + plasma cells in HIV-1 infection
    Article Snippet: .. One hundred microliters of suspension was blocked for nonspecific staining using anti-mouse serum, stained for IgA (PE Miltenyi Biotec, catalog no. 130–113-476), washed thrice, and stained for DNA (SYBRGreen Applied Biosciences). .. IgA-bound (SYBRGreen + , IgA + ) and IgA-unbound (SYBRGreen + , IgA − ) stool bacteria were sorted and pelleted, and DNA was extracted using the QIAamp UCP Pathogen Mini Kit (Qiagen catalog no. 50214).

    Article Title: Gastrointestinal germinal center B cell depletion and reduction in IgA + plasma cells in HIV-1 infection.
    Article Snippet: .. One hundred microliters of suspension was blocked for nonspecific staining using anti- mouse serum, stained for IgA (PE Miltenyi Biotec, catalog no. 130- 113- 476), washed thrice, and stained for DNA (SYBRGreen Applied Biosciences). .. IgA- bound (SYBRGreen+, IgA+) and IgA- unbound (SYBRGreen+, IgA−) stool bacteria were sorted and pelleted, and DNA was extracted using the QIAamp UCP Pathogen Mini Kit (Qiagen catalog no. 50214).

    Suspension:

    Article Title: Gastrointestinal germinal center B cell depletion and reduction in IgA + plasma cells in HIV-1 infection
    Article Snippet: .. One hundred microliters of suspension was blocked for nonspecific staining using anti-mouse serum, stained for IgA (PE Miltenyi Biotec, catalog no. 130–113-476), washed thrice, and stained for DNA (SYBRGreen Applied Biosciences). .. IgA-bound (SYBRGreen + , IgA + ) and IgA-unbound (SYBRGreen + , IgA − ) stool bacteria were sorted and pelleted, and DNA was extracted using the QIAamp UCP Pathogen Mini Kit (Qiagen catalog no. 50214).

    Article Title: Gastrointestinal germinal center B cell depletion and reduction in IgA + plasma cells in HIV-1 infection.
    Article Snippet: .. One hundred microliters of suspension was blocked for nonspecific staining using anti- mouse serum, stained for IgA (PE Miltenyi Biotec, catalog no. 130- 113- 476), washed thrice, and stained for DNA (SYBRGreen Applied Biosciences). .. IgA- bound (SYBRGreen+, IgA+) and IgA- unbound (SYBRGreen+, IgA−) stool bacteria were sorted and pelleted, and DNA was extracted using the QIAamp UCP Pathogen Mini Kit (Qiagen catalog no. 50214).

    Selection:

    Article Title: Colonic spatial single-cell proteomics and murine models link mitochondrial dysfunction to dimeric IgA-secreting plasma cell deficiency in Crohn’s disease
    Article Snippet: .. IgA + and IgG + switched memory B cells (sMBCs) were enriched from colonic LPMNCs using negative selection magnetic-activated cell sorting (MACS; a cocktail of biotin-conjugated monoclonal antibodies against CD2, CD14, CD16, CD36, CD43, CD235a, IgM, and IgD) following the manufacturer’s instructions of the Switched Memory B Cell Isolation Kit (Miltenyi #130-093-617). ..

    FACS:

    Article Title: Colonic spatial single-cell proteomics and murine models link mitochondrial dysfunction to dimeric IgA-secreting plasma cell deficiency in Crohn’s disease
    Article Snippet: .. IgA + and IgG + switched memory B cells (sMBCs) were enriched from colonic LPMNCs using negative selection magnetic-activated cell sorting (MACS; a cocktail of biotin-conjugated monoclonal antibodies against CD2, CD14, CD16, CD36, CD43, CD235a, IgM, and IgD) following the manufacturer’s instructions of the Switched Memory B Cell Isolation Kit (Miltenyi #130-093-617). ..

    Magnetic Cell Separation:

    Article Title: Colonic spatial single-cell proteomics and murine models link mitochondrial dysfunction to dimeric IgA-secreting plasma cell deficiency in Crohn’s disease
    Article Snippet: .. IgA + and IgG + switched memory B cells (sMBCs) were enriched from colonic LPMNCs using negative selection magnetic-activated cell sorting (MACS; a cocktail of biotin-conjugated monoclonal antibodies against CD2, CD14, CD16, CD36, CD43, CD235a, IgM, and IgD) following the manufacturer’s instructions of the Switched Memory B Cell Isolation Kit (Miltenyi #130-093-617). ..

    Article Title: Circulating mucosal-like IgA responses increase with severity of Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome
    Article Snippet: LOX-1 , PE , 15C4 , BioLegend. .. IgA , PE-Cy7 , IS11-8E10 , MACS Miltenyi. .. CD11b , APC-Cy7 , ICRF44 , Biolegend.

    Bioprocessing:

    Article Title: Colonic spatial single-cell proteomics and murine models link mitochondrial dysfunction to dimeric IgA-secreting plasma cell deficiency in Crohn’s disease
    Article Snippet: .. IgA + and IgG + switched memory B cells (sMBCs) were enriched from colonic LPMNCs using negative selection magnetic-activated cell sorting (MACS; a cocktail of biotin-conjugated monoclonal antibodies against CD2, CD14, CD16, CD36, CD43, CD235a, IgM, and IgD) following the manufacturer’s instructions of the Switched Memory B Cell Isolation Kit (Miltenyi #130-093-617). ..

    Cell Isolation:

    Article Title: Colonic spatial single-cell proteomics and murine models link mitochondrial dysfunction to dimeric IgA-secreting plasma cell deficiency in Crohn’s disease
    Article Snippet: .. IgA + and IgG + switched memory B cells (sMBCs) were enriched from colonic LPMNCs using negative selection magnetic-activated cell sorting (MACS; a cocktail of biotin-conjugated monoclonal antibodies against CD2, CD14, CD16, CD36, CD43, CD235a, IgM, and IgD) following the manufacturer’s instructions of the Switched Memory B Cell Isolation Kit (Miltenyi #130-093-617). ..

    other:

    Article Title: Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel
    Article Snippet: 640nm , R1 , IgA , APC , Miltenyi , 130-113-998 , IS11-8E10 , Mouse , IgG1 κ , AB_2733421 , 200 , N/A.



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